Southampton have lost Kyle Walker-Peters to injury in defeat to Wolves.
The defender, who only returned from a four-game lay-off due to a quad problem on Thursday, has now picked up another muscle concern.
He could only manage 72 minutes in defeat to Wolves.
Asked for an update on the 23-year-old, boss Ralph Hasenhuttl said: "It's not good. Hamstring problems again.
"Two games in four days after a long injury is not an ideal thing to do. But we have no alternatives at the moment, so it's a pity."
