La Dea saw their unbeaten run come to an end in the league, with Gasperini furious an equaliser at 2-1 was denied by the match officials.
"We certainly made mistakes and conceding a goal in the opening minute after a bunch of ricochets, much like against Milan and Villarreal, makes it difficult," Gasperini told DAZN.
“However, as in all those cases, the team did recover and was playing with purely one-way traffic. That incident changed the game and I demand an explanation.
“If (Luis) Palomino didn't touch the ball, then why is it offside. If Cristante got the ball, then what difference does it make? Are we kidding, here? If Palomino doesn't touch the ball, then it is not interfering with play, he is behind Cristante.
“We would then have been at 2-2 with 20 minutes to go, it's a very different game with the momentum behind Atalanta. Don't get me wrong, Roma might've won anyway, but that was a transformative moment of the match.
“If Palomino didn't touch the ball, then it's madness to disallow that. Madness. Once again, have we changed the rules and nobody told us? The referee told us Palomino touched it and he was offside. If that's not true, then what happened?
“This sort of thing takes credibility away from the sport. Palomino did not touch the ball at all. This is madness! He didn't touch Cristante either, he was behind him, so not interfering with play.
“I lost 4-1 and come here to talk to you, so why don't the referees come here and explain themselves too? Why not the VAR? They are ruining the credibility of football.
“If they tell me it's a mistake, we're unlucky, but don't try telling me that is the rule. Otherwise it has to be the rule always. What are we talking about here?"
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