Manchester United were beaten 1-0 by FC Copenhagen in the UEFA Youth League.
As the two senior teams met at Old Trafford, their Under-19s also competed on Tuesday.
While the senior Red Devils won 1-0 at home, the youth side were beaten by the same scoreline.
Queyrell Tchicamboud's winner settled the contest for the Danish side at Leigh Sports Village.
Emil Hojlund, brother of Man United forward Rasmus, was playing for Copenhagen in the game.
United did get to give minutes to youngster Kobbie Mainoo, who will be involved with the senior team as he continues his recovery from an ankle injury.