Canada's maple-leaf flag went up and dozens of people sang “O Canada” as Foreign Minister Anita Anand officially opened the country's consulate in Nuuk, which is also the largest city of the icy Arctic island.
Around 50 people gathered for the event and applauded as the flag was hoisted.
Canada and France oppose claims by US President Donald Trump over the Danish autonomous territory.
Greenland Foreign Minister Vivian Motzfeldt said the move highlights shared values and strong ties with Canada.
France says the decision to open its outpost was made when President Emmanuel Macron visited in June.