Hamburg is withdrawing its bid to host Olympic Games in the future after the plans were rejected in a referendum on Sunday, leaving only three candidates from the country.
The vote is binding and mayor Peter Tschentscher said that the governing senate has acted accordingly.
"I have just informed the president of the [German Olympic Sports Confederation] DOSB, Thomas Weikert, and Germany's representative at the [International Olympic Committee] IOC, Michael Mronz, that Hamburg is withdrawing its Olympic bid," Tschentscher said.
With results still being counted, the state electoral chief Oliver Rudolf said a majority of participants opposed the bid to stage the 2036, 2040 or 2044 Summer Olympics in Hamburg.
The unofficial final result was expected later on Sunday.
Around 1.3 million people from the age of 16 were eligible to vote, with turnout at 49.5%.
The figure is lower than the 50.2% who participated in a 2015 referendum on a bid for the 2024 Olympics which had a similar outcome, with 51.6% against Games and 48.4% in favour.
Hamburg was competing with Munich, Berlin and a Cologne-led Rhine-Ruhr region bid to become the German candidate.
The decision on who will bid and for which year will be made by the DOSB on September 26.
Munich's bid was backed by a 66.4% majority in a referendum and Cologne/Rhine-Ruhr by 66%. In Berlin, the local parliament approved the bid, with a referendum not possible there for constitutional reasons.
DOSB chairman Otto Fricke had hoped for a positive result from Hamburg but that did not happen.
Tschentscher had named Olympics a big opportunity for the northern German port city, citing economic reasons and new Olympic guidelines under which thwe Games have to adapt to a host city and not vice versa as in the past.
Supporters highlight the use of existing venues, an expected profit and an important boost for the city's infrastructure. Critics cited out uncertainties about, that the money should be spent for more pressing projects, and environmental concerns.
Germany hosted summer Olympics 1936 in Berlin and 1972 in Munich.
