Southampton boss Ralph Hasenhuttl confirmed Kyle Walker-Peters is due for another scan on his hamstring injury.
The right-back was missing as the Saints secured a 1-1 draw against Arsenal in the Premier League on Sunday.
Lycano was involved as a full-back in the game, before Hasenhuttl tweaked his formation to put Moi Elyounoussi in that position.
Post-game, the Austrian has said: “We don't have another update yet. We will have to wait until next week until we have another scan and then we'll know about it."
He had stated pre-game that the Walker-Peters injury was “a big one" and could keep him out for a while.
The Saints will be hoping he is available for their remaining games before the World Cup break.