Holders Bayern Munich have been drawn away to second-tier newcomers VfL Osnabrück in the German Cup first round in September.
Osnabrück recently won the third division.
The draw on Saturday was held at the German Football Museum in Dortmund and carried out by retiring top referee Deniz Aytekin. Borussia Dortmund will travel to fifth tier HEBC Hamburg also on September 1 or 2.
Dortmund, second in the Bundesliga in the season just finished, host double winners Bayern Munich in the Franz Beckenbauer Super Cup on August 22 - the weekend when the rest of the German Cup first-round games are taking place.
VfB Stuttgart, beaten in the Cup final by Bayern last month and 2025 winners, face a trip to third division Hansa Rostock.
Bayer Leverkusen, the 2024 Cup winners, go to third tier Wehen Wiesbaden.
First-round fixtures:
SC St Tönis v Eintracht Frankfurt
Erzgebirge Aue v Hoffenheim
Eintracht Braunschweig v Union Berlin
Eintracht Trier v RB Leipzig
HEBC Hamburg v Borussia Dortmund
TSV Schott Mainz v Borussia Mönchengladbach
Fortuna Düsseldorf v Freiburg
Rot-Weiss Essen v St Pauli
SG Sonnenhof Grossaspach v Arminia Bielefeld
SSV Jeddeloh II v Heidenheim
SV Wehen Wiesbaden v Bayer Leverkusen
Halle v Schalke 04
Energie Cottbus v Augsburg
VfB Krieschow v Mainz
Greuther Fürth v VfL Bochum
SV Hemelingen v Hanover
Preussen Münster v Karlsruhe
Saarbrücken v Hertha Berlin
Viktoria Cologne v Nuremberg
MSV Duisburg v Elversberg
VfL Osnabrück v Bayern Munich
Lüneburger SK Hansa v Werder Bremen
Verl v SV Hamburg
Hansa Rostock v VfB Stuttgart
Carl Zeiss Jena v Darmstadt
Bahlingen v Magdeburg
1860 Munich v Holstein Kiel
Waldhof Mannheim v Kaiserslautern
Westfalia Rhynern v Dynamo Dresden
Phönix Lübeck v Paderborn
Altglienicke v Wolfsburg
Würzburger Kickers v Cologne
